Association of DNA Polymerase (pol ) with Ku and Ligase IV: Role for pol in End-Joining Double-Strand Break Repair
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چکیده
Association of DNA Polymerase (pol ) with Ku and Ligase IV: Role for pol in End-Joining Double-Strand Break Repair Kiran N. Mahajan, Stephanie A. Nick McElhinny, Beverly S. Mitchell, and Dale A. Ramsden* Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, and Departments of Pharmacology and Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
منابع مشابه
Microhomology-mediated DNA strand annealing and elongation by human DNA polymerases λ and β on normal and repetitive DNA sequences
'Classical' non-homologous end joining (NHEJ), dependent on the Ku70/80 and the DNA ligase IV/XRCC4 complexes, is essential for the repair of DNA double-strand breaks. Eukaryotic cells possess also an alternative microhomology-mediated end-joining (MMEJ) mechanism, which is independent from Ku and DNA ligase 4/XRCC4. The components of the MMEJ machinery are still largely unknown. Family X DNA p...
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Non homologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA ligase (Lig), polymerase (Pol), phosphoesterase (PE) and Ku from a mesophillic archaeon, Methanocella paludicola (Mpa). Mpa ligase has limited DNA ...
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DNA double-strand breaks are repaired by multiple mechanisms that are roughly grouped into the categories of homology-directed repair and non-homologous end joining. End-joining repair can be further classified as either classical non-homologous end joining, which requires DNA ligase 4, or "alternative" end joining, which does not. Alternative end joining has been associated with genomic deleti...
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